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KMID : 0364819850230040309
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Korean Journal of Microbiology
1985 Volume.23 No. 4 p.309 ~ p.314
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Genetic Recombination by Protoplast Fusion of Cellulomonas sp CS 1-1
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Kim Byung-Hong
Lee Hak-Joo
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Abstract
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1
Auxotrophic mutants of a cellulolytic baterium Cellulomonas sp. CS 1-1 were grown at 30°C for 6 hr using a complete medium containing 0.5M sucrose and for another 90 min after addition of 0.3 U/ml penicillin G, and were protoplasted by 0.2 mg/ml lysozyme for 2 hr. Prototrophic recombinants were obtained at the rates of 10-3 to 10-5 by fusing the protoplasts in the presence of 40% polyethyleneglycol 3350. Nystatin could be used to eliminate fungal contamination during the regeneration of the plotaplasts.
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KEYWORD
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